ABSTRACT
Cre/LoxP site specific recombinase system is an effective tool for gene recombination in the body to produce specific gene deletion or inversion.The study of gene function is the experimental basis for the development of experimental animal models.Study the Cre/LoxP system,compare the different function characteristics of mouse models for Cre recombinase system in the research of ophthalmology,epecially in the tissue of retina,such as retinal pigment epithelium (RPE) cells,Müller cells and photoreceptor cells,get the Cre transgenic mouse model to establish proper experimental animal models of ophthalmology will lay a solid foundation for the research of the lack of single gene for a particular organization study.Here,the research progress of Cre/LoxP system,the Cre transgenic mouse models and eye specific Cre recombinant enzyme model mice will be summarized.
ABSTRACT
Objective To explore the ideal postoperative nursing methods of repair the lacerateon of lacrimal canaliculus. Methods Using pigtail probe to repair the lacerateon of lacrimal canaliculus in 49 patients, observed the postoperative nursing points in different clinical phases. Results There were 2 patients had the anstomotic stoma split, 1 patient had the canaliculitis. There were 46 patients had successful extubation. Conclusion The Worst lacrimal probe is an satisfactory and reliable method to repair the lacerateon of lacrimal canaliculus. The detailed interpretation and the skillful nursing care are the guarantee for successful operation.